SEEP Eco Sponges Washing Up - Compostable Kitchen Sponge - Cleaning Sponges Made Using Loofah - Sponge Scourer - 4 x Pack Multipack

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The results indicated that all ewes ovulated within 58 hours after removal of CIDR ® OVIS and administration of eCG. Synchronising ewes using sheep sponges and PMSG is probably the most common method of advancing the breeding season. From the perspective of shortening the breeding cycle and saving breeding costs, it is recommended to use the FGA sponge for 11 days, namely, the protocols of Groups I, II, and III. Although there was no significant difference in the total percentage of estrous ewes in different schemes, the total percentage of estrous ewes in the scheme of Group I was only 70%, whereas it was both more than 80% in the schemes of Groups II and III. Therefore, two schemes of Groups II and III could be recommended. Conception and Lambing of Ewes There are a number of different programmes that can be used to help ewes come into season early and produce a good crop of lambs. Two of the most common programmes are progestogen impregnated sponges with PMSG and secondly melatonin implants. Sheep sponges and PMSG Introduce rams (35 days after implantation). Peak mating activity will occur 25-35 days after introduction of rams.

The co-author of the first study on this unusual ecosystem, Professor Antje Boetius, says, 'This is a unique ecosystem, and we have never seen anything like it before in the high central Arctic. Some farmers will ‘sponge’ their ewes to ensure a more compact lambing down period. It is a very interesting process that involves the inserting of small sponges, which have been infused with progestogen into the ewe’s vagina. Over time, this allows organisms which feed on these microbes to arrive and survive, followed by other animals including predators. This creates areas that are significantly more diverse than the surrounding ocean, with one study describing them as 'oases' of the Arctic. Growing early spring grass is essential for the system to work well and to reduce concentrate usage. JW: funding acquisition, project administration, supervision, conceptualization, methodology, investigation, writing—review, and editing. XY: investigation, data analysis, writing—review, and editing. YB: investigation, data analysis, and writing—original draft preparation. JY and XZ: investigation and software. LZ: investigation. All authors have read and agreed to the published version of the manuscript. FundingMicrobial DNA was extracted from sponge samples using the E.Z.N.A. ® Soil DNA Kit (Omega Bio-tek, Norcross, GA, U.S.) following the manufacturer’s protocols. The V1-V9 regions of the bacterial 16S rRNA gene were amplified by PCR (95°C for 2 min, followed by 27 cycles at 95°C for 30 s, 55°C for 30 s, and 72°C for 60 s, and a final extension at 72°C for 5 min) using primers 27F 5’-AGRGTTYGATYMTGGCTCAG-3’ and 1492R 5’-RGYTACCTTGTTACGACTT-3’ ligated with an eight-base barcode unique to each sample. PCR reactions were performed in triplicate 20 μL mixture containing 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of each primer (5 μM), 0.4 μL of FastPfu Polymerase, and 10 ng of template DNA. Amplicons were extracted from 2% agarose gels and purified using the Axy Prep DNA Gel Extraction Kit (Axygen Biosciences, Union City). SMRT bell libraries were prepared from the purified amplicons by blunt ligation according to the manufacturer’s instructions (Pacific Biosciences). Purified SMRT bell libraries from the pooled and barcoded samples were sequenced on a single PacBio Sequel II cell by Shanghai Biozeron Biotechnology Co. Ltd (Shanghai, China). Avoidance of stress is also vital. If you can avoid using dogs at sponging, at removal and in between that helps.

Sitting high in the Arctic circle, the Langseth Ridge varies from around 500 metres to over five kilometres deep. During the time that Rome was becoming one of the world's first superpowers over 2,000 years ago, a seep community was thriving on the ridge. Primary productivity in the overlying water provides less than one percent of the sponges' carbon demand, with fossil matter contributing a significant part of the remaining amount. Thus, this sponge garden may be a transient ecosystem, but it is rich in species, including soft corals.' Not directed at anyone in particular but I sometimes notice at friends or family's houses that the sponge is usually kept at the bottom of the sink or floating in a bowl of cold, greasy washing up water. This has allowed the sponges to grow substantially compared with others in nearby waters, with those at the centre of the fossil community growing largest. The organisms also show signs of reproducing, suggesting an abundance of nutrients and energy being available. You should consult your veterinary surgeon about which programme is most suitable for your farm and your sheep, and follow veterinary advice at all times.”Studies have shown that local vaginitis and alterations of vaginal flora were present after the ewes were treated with the intravaginal sponge ( 40, 41). Gram-negative bacteria and gram-positive bacteria appeared in the vagina after using the vaginal progestogen estrous device ( 42). Manes et al. revealed that, after the use of intravaginal sponges, the vaginal wall of treated ewes showed epithelial hyperplasia and hypertrophy, and perivascular inflammatory cell infiltration ( 43). These histological and cytological changes are similar to those which occurred in other inflammatory processes of the reproductive tract and could have a negative impact on the fertility of ewes for estrus synchronization. Martinez-Ros et al. have indicated that abnormal vaginal discharge occurred after the ewes were administered with intravaginal sponges for estrus synchronization. More ewes had purulent and hemorrhagic discharges, which were administered by the intravaginal sponge for the long term ( 44). Purulent and hemorrhagic discharges were typical features of vaginitis. Vaginitis has little effect on the fetus in the early stages of pregnancy but may cause fetal infection due to the presence of vaginal bacteria during the birth of lambs. Therefore, to reduce the inflammatory reaction of the vagina of ewes, antibiotics are usually used when using vaginal progestogens estrous device to induce estrous synchronization ( 45). The amount of mucus ( P< 0.01) and odor ( P< 0.001) were reduced in the antibiotic group vs. the control group when the ewes were treated by the vaginal device for estrous synchronization ( 46). In this study, vaginal suppositories were used for 11 and 13 days, which are long-term programs, so antibiotics were used to prevent the occurrence of vaginitis in ewes. Antibiotics can prevent the occurrence of vaginitis to a certain extent, but they cannot completely inhibit the occurrence of vaginitis. The results of this study are consistent with previous research. Although antibiotics were used, some ewes still showed vaginitis, and there was no significant difference in the vaginal inflammation rate of ewes among five protocols of estrous synchronization. Drug Cost of Estrus Synchronization

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmars.2023.1243952/full#supplementary-material Yan Wang 1,2,3† Lin Gong 1,2† Zhaoming Gao 4* Yong Wang 5 Feng Zhao 1,2 Lulu Fu 1 Xinzheng Li 1,2,3,6* Compost: Cut up the sponge (this speeds up composting) and place it in a home compost of food waste bin To achieve good results with synchronising ewes, ewes should be weaned for at least 6 weeks and be in a body score 3.5-4.0 at sponging.

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You will get most of them lambing together. Sheep served on the same day tend to lamb in 48 hours give or take 48 hours. You'll get a small batch served on first repeat. They will be served over a 4 day period, so you get a few ewes lambing over a broader timescale. The second repeat are served over a week so with an even broader range of lambing times for the smallest number of ewes. On the basis of the previous studies, this study hypothesized that (1) the effects of PMSG and GnRH are similar on the synchronization of estrus in Hu ewes and that (2) the FGA vaginal sponge of long-term use (11 and 13 days) has the same effect on the synchronization of estrus in Hu ewes. The objective of this study is to obtain the most practical protocol by comparing five treatments of estrous synchronization for improving Hu sheep breeding. Materials and Methods Animals

for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China My thinking is that, logically, anything that scrubs your plates/glasses/cutlery should also be clean enough to put in your mouth. All I see with the floaty or sink-bottom-dreg-filled sponge is a bacteria factory (especially if it's a meat eating household) but it is so common, I wonder if I'm the odd one here? Replacing plastic sponges with these plant based ones means not adding microplastics into your home and into our waterways. Also less plastic is being produced from extracted oil so is a step away from fossil fuel reliance, and a step towards more a more sustainable way of living. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Publisher's NoteNot necessary, but it is advised, so why not. It's the smell that does the job apparently. We've teased by putting the tup in a pen next to the sheep before now, but that comes with a huge health warning obviously i.e. I don't know how good your pens are or how good your tup is at jumping out of them... For exact timing of insemination, the technique (cervical or laparoscopic) and quality of semen used (fresh or frozen) should be taken into consideration. The percentage of estrous ewes treated with progestogen sponge for a long term (14 days) was significantly higher than that of ewes treated for 7 and 10 days ( P< 0.05) ( 17). In this study, there was no significant difference in the total percentage of estrous ewes treated with different protocols of estrus synchronization, and there was no significant difference in the proportion of estrous ewes in any one of the five time periods within 72 h after withdrawal of the sponge among five protocols. It may be because the 11 and 13 days are both long-term treatments, and the time difference of 2 days is not enough long to cause the difference in the percentage of estrous ewes. The results proved that the second hypothesis of this study was established. This research was funded by Key Research and Development Plan Program of Hebei Province, China (Grant Nos: 18236609D and 20326629D), Innovative Capacity Improvement Program of Hebei Province, China (Grant No: 20536601D), and the Excellent Going Abroad Experts' Training Program in Hebei Province, China. Conflict of Interest Regarding the loss rate of sponge of ewes, the results of previous studies are not consistent. Fonseca et al. ( 37) and Husein et al. ( 38) have shown that the longer the treatment time of the vaginal sponge in ewes, the more likely the sponge was lost, indicating that the treatment time is one of the influencing factors for the loss of the sponge. However, Swelum et al. ( 39) concluded that there was no significant difference in the loss rate of sponge in ewes with a different treatment time of the intravaginal estrus device (3, 6, 9, and 12 days). The results of this study showed that there was no significant difference in the loss rate of sponge of ewes among the five treatments of estrous synchronization, which was consistent with the latter. However, it should be noted that the loss rate of the sponge showed an upward trend when the sponge was used for 13 days.