Acid/Flux Brush, Tin Ferrule, Horsehair, 5-3/4" Length, 5/16" Width(Pack of 30 Pieces)

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W. Foster, K. Miyazawa, T. Fukuma, H. Kusumaatmaja and K. Voiotatchovsky, Nanoscale, 2020, 12, 5452–5463 RSC.

A 2013 national survey [footnote 2] found that only a quarter of children benefited from having their teeth brushed before they were 6 months of age, whereas about 50% had commenced between 6 months and one year of age. Overall about 90% of children (aged 5 to 8 years) are reported as having started toothbrushing by 2 years of age [footnote 2]. Overall, 77% of 12-year-olds and 81% of 15-year-olds reported that they brushed their teeth twice daily or more. Mouthwash was the most common aid other than a toothbrush (manual or powered) and toothpaste. As expected, the use of mouthwashes, dental floss (the only interdental cleaning method investigated) and sugar-free gum was generally higher in older children. Approximately 40% of the school children surveyed used a powered toothbrush. Oral hygiene principles for oral health T. Fukuma, M. Kimura, K. Kobayashi, K. Matsushige and H. Yamada, Rev. Sci. Instrum., 2005, 76, 053704 CrossRef. The addition of 1 mM CaCl 2 caused a distinct change in both topographical and mechanical properties of the brush/electrolyte interface. In the presence of Ca 2+ ions, pAA-Cys5 brushes became smoother, which could be characterized by the very small roughness, RMS Ca = 0.15 nm ( Fig. 3b) with no characteristic feature ( Fig. 3e). The 3D Δ f map and its xz cross-section ( Fig. 4b and e) were smoother, and the increase in Δ f was much sharper ( Fig. 4h) than the corresponding values obtained in the absence of Ca 2+ ions, RMS(Δ f) = 0.37 kHz at Δ z = 0 nm ( Fig. 4j), indicating that pAA-Cys5 brushes are uniformly compacted by the presence of Ca 2+ ions. The most remarkable difference was observed when 1 mM CdCl 2 was added to the electrolyte. The surface topography became very rough (RMS Cd = 1.47 nm, Fig. 3c), displaying hemi-ellipsoidal features. The autocorrelation analysis suggested that these protrusions had a similar size and assembled randomly ( Fig. 3f). Accordingly, the 3D Δ f map became highly heterogeneous ( Fig. 4c), and the xz cross-section ( Fig. 4f) clearly indicates the coexistence of stiff protrusions and defects. The heterogeneity of the mechanical landscape was clearly visible from a broad distribution of the Δ f–Δ z curves, resulting in RMS(Δ f) = 1.90 kHz at Δ z = 0 nm ( Fig. 4j). These data suggest that pAA-Cys5 brushes “collapsed” in the presence of Cd 2+ ions, where water might not be a good solvent for pAA-Cys5. T. Kaindl, H. Rieger, L. M. Kaschel, U. Engel, A. Schmaus, J. Sleeman and M. Tanaka, PLoS One, 2012, 7, e42991 CrossRef CAS.

J. Rühe, M. Ballauff, M. Biesalski, P. Dziezok, F. Gröhn, D. Johannsmann, N. Houbenov, N. Hugenberg, R. Konradi, S. Minko, M. Motornov, R. R. Netz, M. Schmidt, C. Seidel, M. Stamm, T. Stephan, D. Usov and H. Zhang, Adv. Polym. Sci., 2004, 165, 79–150 CrossRef. Among the various techniques, atomic force microscopy (AFM) is commonly used to investigate the mechanical properties of polymer brushes via nanoindentation. 13,14 Recently, Fukuma et al., developed three-dimensional scanning force microscopy (3D-SFM) based on frequency modulation AFM (FM-AFM), 15 which has been utilized to construct force maps of material surfaces. During the measurement process, a cantilever tip scans in the vicinity of the interface both parallel and perpendicular to the surface, and the frequency shift of the oscillating cantilever is recorded in 3D space. This technique has been successful in visualizing the 3D force maps of the surfaces of various materials in water, e.g., minerals, 16,17 graphene, 18 graphite, 19 and supported phospholipid bilayers. 20 A key advantage of this technique is that it can be used to investigate the density distribution of water at the solid/water interface. By exploiting this unique functionality, 3D-SFM has been applied to observe the ammonia-mediated hydration of poly(vinyl alcohol) coated surfaces. 21 However, to our knowledge, no experimental studies have demonstrated the potential of 3D-SFM to detect dynamic modulations of hydrated polymer brushes driven by external chemical stimuli. d Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Osaka 560-8531, Japan Fig. 3 2D-FM-AFM topography measurements of pAA-Cys5 polymer brushes. (a–c, top) 2D images of polymer brushes in (a) 100 mM NaCl, (b) 100 mM NaCl + 1 mM CaCl 2, and (c) 100 mM NaCl + 1 mM CdCl 2, and (bottom) line profiles obtained at the dashed lines in 2D images. Scale bars: 100 nm. (d–f) Autocorrelation maps of the topography images shown in (a–c); (d) 100 mM NaCl, (e) 100 mM NaCl + 1 mM CaCl 2, and (f) 100 mM NaCl + 1 mM CdCl 2. Scale bars: 100 nm. (f, inset) The line profiles along the major and minor axes of the central peak and their best Gaussian fits. The profiles are shifted vertically for the clarity. X. Xu, D. Mastropietro, M. Ruths, M. Tirrell and J. Yu, Langmuir, 2019, 35, 15564–15572 CrossRef CAS.

This guidance is issued jointly by the Department of Health and Social Care, the Welsh Government, the Department of Health Northern Ireland, Public Health England, NHS England and NHS Improvement, and with the support of the British Association for the Study of Community Dentistry. Fig. S5 † shows the overlayed force curves extracted from Fig. 4g–i. Similar to the corresponding Δ f curves ( Fig. 4g), the force curves in NaCl buffer overlap with one another, showing a slow increase to F = 3.5 nN at the interface with the onset of F increase at Δ h = 4 nm (Fig. S5a †). This is reasonable because the interface between hydrated polyelectrolyte brushes and the electrolyte is diffusive. 52,53 In the presence of an additional 1 mM CaCl 2, the onset of F increase was observed at a shallower position (Δ h = 2 nm), and F increased to F = 2 nN at the interface (Fig. S5b †). A minor deviation in the force curves indicated that the pAA-Cys5 brushes were uniformly compacted and therefore stiffened following the binding of Ca 2+ ions. Interestingly, the force curves in the presence of Cd 2+ ions showed a much more pronounced deviation (Fig. S5c †). Both the onset of F increase and the F value at Δ h = 0 nm exhibited wide distributions, implying that pAA-Cys5 brushes formed stiff domains on the surface of the phospholipid monolayer. The formation of collapsed domains in the presence of Cd 2+ ions suggested that the compaction-induced dehydration of pAA-Cys5 brushes altered the affinity of the solvent (water) to monomers. Further investigations, including in situ observations of changes in the viscoelasticity of pAA-Cys5 using 3D-SFM or other techniques ( e.g., QCM-D) would provide further insights into the ion-specific, dynamic modulation of polyelectrolyte brushes. Conclusions In this study, we investigated the modulation of topography and mechanical properties of supported lipid monolayers incorporating lipids functionalized with linear poly(acrylic acid) chains with cysteine side chains (DOPE-pAA-Cys5) induced by different metal ions. We found that the topographical profile of pAA-Cys5 brushes obtained by 2D-FM-AFM in the electrolyte containing no divalent cation (100 mM NaCl, 10 mM Tris–HCl, pH = 7.4) was very homogeneous, whose RMS 0 = 0.23 nm ( Fig. 3a). In fact, autocorrelation analysis of Δ h showed no clear feature ( Fig. 3d). The mechanical landscape, characterized by the 3D Δ f map ( Fig. 4a) and its xz cross-section ( Fig. 4d), was also highly uniform, suggesting that the hydrated pAA-Cys5 brushes are stretched into the bulk electrolyte. The slow increase in the Δ f–Δ z curves ( Fig. 4g) and a very low RMS(Δ f) = 0.24 kHz at Δ z = 0 nm ( Fig. 4j) implied a smooth transition from the polymer to the bulk electrolyte. Fig. 2 Fluorescence image of a supported membrane (DOPC/DOPE-pAA-Cys5/Texas Red DHPE = 94 : 5 : 1 mol%) on a glass cover slip coated with an ODTMS monolayer, verifying the formation of a uniform lipid monolayer incorporating DOPE-pAA-Cys5. Scale bar: 50 μm. Introduction Stimuli-responsive polymers have attracted interest for use in various drug delivery and microencapsulation applications because these substances can protect and/or release materials in response to the surrounding environment. 1 In particular, polyelectrolyte brushes have been grafted on surfaces to give the materials adaptable functionalities. In these cases, the physical properties of the surface can be modulated based on the electrostatic properties of the external media ( e.g., pH and salt concentrations). 2–4 The structures and mechanical properties of polymer brushes have been measured experimentally using X-ray/neutron reflectivity, 5,6 quartz crystal microbalance with dissipation (QCM-D), 7,8 tribology, 9,10 and microinterferometry. 11,12 where δ ind is the indentation depth, μ is Poisson's ratio, and R is the tip radius. However, it is well established that AFM indentation data for a thin film on a stiff substrate cannot be treated with a conventional Hertz model because the film deformation is limited under a high load. 46,47 If the film is softer than the underlying solid substrate, one can analytically calculate Young's modulus of very thin, soft films using a transition function that linearly connects the influence of two elastic layers, i.e., the film and the substrate 48,49 as shown in eqn (6),Fig. 1 Chemical structure of DOPE-pAA-Cys5 and schematic illustration of the experimental procedure whereby a lipid monolayer is formed from a suspension of small unilamellar vesicles (SUV) and an N-octadecyltrimethoxysilane (ODTMS) monolayer on silicon wafers. T. Fukuma, Y. Ueda, S. Yoshioka and H. Asakawa, Phys. Rev. Lett., 2010, 104, 016101 CrossRef PubMed. Toothbrushing is important throughout life. The overall goal is to achieve and maintain good oral hygiene as follows: N. D. Spencer and W. T. Tysoe, The Cutting Edge of Tribology, World Scientific Publishing, 2015 Search PubMed. H. Asakawa, S. Yoshioka, K.-i. Nishimura and T. Fukuma, ACS Nano, 2012, 6, 9013–9020 CrossRef CAS PubMed.