Mouse Heart

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I loved reading of such worlds in Joan Aiken's books when I was a child, and the author doffs her cap to Aiken at the back of the book. Your words, like your reviews, bring books to life and give them a voice before the front cover is even turned. We found that most sister cell pairs show matched high or low GFP intensity levels at the end of the observation period (38 out of 39, Figure 5I, Figure 5—figure supplement 3A–D and Figure 5—source data 1). We found that the GFP level varied linearly with cTnnT level ( Figure 3D), although considerable variability of GPF levels was observed within each cTnnT+ and cTnnT- cell populations.

To assess how the initial cardiogenic region transforms into a HT and differentiates, we first analyzed Nkx2. Ils1 cre/+; Rosa26Rtdtomato +/- embryos at different stages from cc up to heart looping (n = 10) and assessed the appearance of GFP and tdtomato double-positive domains in the HT.Here, we show the relevance of differentiation timing regulation during heart tube formation and its coordination with morphogenesis at the tissue level.

embryos from transversal HT showing low GFP level prior to cardiomyocyte differentiation and increase in GFP intensity level during the stages cardiomyocytes undergo differentiation. As expected, we observed GFP-low cells located adjacent to GFP-high cells in the boundary zone ( Figure 6H and Video 14). Thus, cardiac power was reduced to 50% of its maximum value when stimulated at approximately 500 beats. Importantly, they retain a columnar shape typical of weak cTnnT+ and cTnnT- cells located at the boundary zone (see Figure 2F’’ and Figure 3B’).The temporal allocation of the morphogenetic phase after cc differentiation allows the formation of the HT while simultaneously providing an incipient cardiac function essential for the organization of embryonic circulation. LV pressure was measured with a micromanometer connected to a small steel cannula inserted through the apex of the heart. Further studies will be required to assess how this temporal pause of cardiac differentiation is regulated.

This study further confirmed that SHF differentiation at the arterial pole is initiated when the HT is about to close dorsally but not before ( Figure 9C).

Left/Right (L/R) asymmetry therefore exists within the cardiac crescent, prior to any detectable cardiac contraction, and BMP/SMAD1 signaling in the lateral plate mesoderm may be involved in this asymmetry ( Furtado et al. Finally, cardiac differentiation resumes contributing new cardiomyocytes from the splanchnic mesoderm to the arterial pole (prospective RV) and the dorsal closure of the HT. Sun Q, Wagg CS, Güven B, Wei K, de Oliveira AA, Silver H, Zhang L, Vergara A, Chen B, Wong N, Wang F, Dyck JRB, Oudit GY, Lopaschuk GD. It means that she can make connections, will outrun pursuers, and then show her Mouse Heart to those she cares for. These measurements estimate that midline convergence of the splanchnic mesoderm takes approximately 5–7 hr from the late cc stage until the open HT stage ( Figure 4—figure supplement 2).

Our results show the feasibility of live time-lapse analysis of mouse HT formation and reveal that splanchnic mesoderm displacement, at least in part by sliding over the endoderm, is an essential aspect of HT morphogenesis. It remains, however, unclear whether this effect is secondary to an initial defect in foregut development that is observed in this mutant. We next studied cardiac differentiation dynamics during subsequent stages when the cc transforms into the HT by extensive morphogenesis.As Mouse’s investigation leads her ever closer to their true, deadly nature, can she outwit them without losing everything she holds dear? cre/+; Rosa26Rtdtomato +/- embryos, tdtomato labeling is also observed in the endocardium and endothelial cells ( Stanley et al.