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a b c d e f g h i j Drmanac, R.; Sparks, A. B.; Callow, M. J.; Halpern, A. L.; Burns, N. L.; Kermani, B. G.; Carnevali, P.; Nazarenko, I.; etal. (2009). "Human Genome Sequencing Using Unchained Base Reads on Self-Assembling DNA Nanoarrays". Science. 327 (5961): 78–81. Bibcode: 2010Sci...327...78D. doi: 10.1126/science.1181498. PMID 19892942. S2CID 17309571. Another advantage of DNA nanoball sequencing include the use of high-fidelity Phi 29 DNA polymerase [10] to ensure accurate amplification of the circular template, several hundred copies of the circular template compacted into a small area resulting in an intense signal, and attachment of the fluorophore to the probe at a long distance from the ligation point results in improved ligation. [2] Disadvantages [ edit ] No buying guide would be complete without at least mentioning price. Thankfully most brands make models at different price points and you can rest assured there is decent performance on offer across the price spectrum. It is also worth considering that personalized balls cost a little more money. Once a single-stranded circular DNA template is created, containing sample DNA that is ligated to two unique adapter sequences has been generated, the full sequence is amplified into a long string of DNA. This is accomplished by rolling circle replication with the Phi 29 DNA polymerase which binds and replicates the DNA template. The newly synthesized strand is released from the circular template, resulting in a long single-stranded DNA comprising several head-to-tail copies of the circular template. [10] The resulting nanoparticle self-assembles into a tight ball of DNA approximately 300 nanometers (nm) across. Nanoballs remain separated from each other because they are negatively charged naturally repel each other, reducing any tangling between different single stranded DNA lengths. [2] DNA nanoball creation and adsorption to the patterned array flowcell DNA nanoball patterned array [ edit ] Publication March 8, 2021 Barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel in situ analyses

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Adapter DNA sequences must be attached to the unknown DNA fragment so that DNA segments with known sequences flank the unknown DNA. In the first round of adapter ligation, right (Ad153_right) and left (Ad153_left) adapters are attached to the right and left flanks of the fragmented DNA, and the DNA is amplified by PCR. A splint oligo then hybridizes to the ends of the fragments which are ligated to form a circle. An exonuclease is added to remove all remaining linear single-stranded and double-stranded DNA products. The result is a completed circular DNA template. [2] Rolling circle replication [ edit ]

Chrisey, L.; Lee, GU; O'Ferrall, CE (1996). "Covalent attachment of synthetic DNA to self-assembled monolayer films". Nucleic Acids Research. 24 (15): 3031–9. doi: 10.1093/nar/24.15.3031. PMC 146042. PMID 8760890. FFFFFFFFFFFGFGFFFFFF;FFFFFFFGFGFGFFFFFF;FFFFGFGFGFFEFFFFFEDGFDFF@FCFGFGCFFFFFEFFEGDFDFFFFFGDAFFEFGFF TGTCTACCATATTCTACATTCCACACTCGGTGAGGGAAGGTAGGCACATAAAGCAATGGCAGTACGGTGTAATACATGCTAATGTAGAGTAAGCACTCAG Are you very much a classic white ball player, or someone open to playing something a little different as the golf ball world evolves? There's more choice than ever in this regard and if you are reading this guide, chances are you want a slightly different color or look to your golf ball. This DNA Fidget Ball offers a great feel, plus it is irresistibly "grabbable", almost impossible to put down.

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Fullwood, M. J.; Wei, C.-L.; Liu, E. T.; Ruan, Y. (2009). "Next-generation DNA sequencing of paired-end tags (PET) for transcriptome and genome analyses". Genome Research. 19 (4): 521–32. doi: 10.1101/gr.074906.107. PMC 3807531. PMID 19339662. a b Blanco, Luis; Bernad, Antonio; Lázaro, José M.; Martin, Gil; Garmendia, Cristina; Margarita, M; Salas (1989). "Highly efficient DNA synthesis by the phage phi 29 DNA polymerase. Symmetrical mode of DNA replication". The Journal of Biological Chemistry. 264 (15): 8935–40. doi: 10.1016/S0021-9258(18)81883-X. PMID 2498321. Huang, Jie; Liang, Xinming; Xuan, Yuankai; Geng, Chunyu; Li, Yuxiang; Lu, Haorong; Qu, Shoufang; Mei, Xianglin; Chen, Hongbo; Yu, Ting; Sun, Nan; Rao, Junhua; Wang, Jiahao; Zhang, Wenwei; Chen, Ying; Liao, Sha; Jiang, Hui; Liu, Xin; Yang, Zhaopeng; Mu, Feng; Gao, Shangxian (2017). "A reference human genome dataset of the BGISEQ-500 sequencer". GigaScience. 6 (5): 1–9. doi: 10.1093/gigascience/gix024. ISSN 2047-217X. PMC 5467036. PMID 28379488. The FISSEQ technology has been licensed to ReadCoor Inc., a start up company spun out of the Wyss Institute, which will commercialize it as a new generation sequencing platform, allowing researchers to perform high throughput RNA sequencing and obtain the cellular locations of multiple RNAs simultaneously in intact cell and tissue samples of their choice.An avid player, Chris currently maintains a handicap index of 2.4 and has a career-low round of 66, which he has shot on three occasions. He lives about 20 miles north of Atlanta in Roswell, Georgia, with his wife, Stacey, and is a member at Atlanta National Golf Club. This low-compression Wilson ball promises extreme distance through a hard ionomer cover material that optimizes trajectory for maximum yardage and roll. And from our testing, we can vouch that this ball certainly delivers. The enhanced aerodynamic dimple pattern really does add to the power potential from the tee and we were so impressed by its distances that we have included it in our best distance golf balls guide. An updated reference human genome dataset of the BGISEQ-500 sequencer". GigaDB . Retrieved 22 March 2017. As its name might suggest, the Wilson Duo Soft+ is the softest golf ball in the current Wilson range. In fact, its compression rating of 35 is among the lowest of any golf ball that’s been manufactured in recent years. The Duo Soft+ features a two-piece construction and a surlyn cover. That said, while surlyn is significantly less responsive than urethane, which is also far more expensive, the Duo Soft+ provides better greenside control than what players might normally expect from a two-piece ball. Which aspect of the game is most important to you? Are you looking for maximum distance or something that allows your short game to shine around the greens?

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Urethane-covered balls offer the best spin control and feel but do cost more. Experiment with each to see if you notice enough of a difference to justify the extra cost, or whether the best mid-price golf balls might be an option. Porreca, Gregory J (2010). "Genome sequencing on nanoballs". Nature Biotechnology. 28 (1): 43–4. doi: 10.1038/nbt0110-43. PMID 20062041. S2CID 54557996. The biggest difference between the two balls is that the R model’s urethane cover is completely unpainted, which is a golf industry first. Wilson maintains that the unpainted ball produces a stronger ball flight on full shots, more friction and spin on short shots, and increased accuracy. In our testing, the first two claims were realized, with the ball flying as far as some of the best distance golf balls on the market. Improved accuracy, however, was difficult to discern. The 2023 Wilson Duo Soft is dubbed by Wilson to be the 'world's softest golf ball' and with a compression rating of 40, it's hard to argue against that. Akin to many of the best soft golf balls on the market, the Wilson Duo Soft is an ionomer covered golf ball that has been designed to allow for maximum energy return, meaning this ball flies further and launches higher with much less effort. We found this was the case when we took it out on the course, where we found it delivered towering, high trajectories with every club in the bag. Wilson has also implemented its new V-COR construction to create a core that delivers increased velocity and distance gains through the bag. Additionally, a proprietary painting process ensures the finish on each ball is perfect and that every Staff Model ball performs as intended on the course. Finally, it comes with a seamless 362-dimple pattern which helps to produce a flatter, more controlled trajectory, which makes the Wilson Staff Model an excellent option for golfers who often find themselves playing in windy conditions.The data generated from the DNA nanoballs is formatted as standard FASTQ formatted files with contiguous bases (no gaps). These files can be used in any data analysis pipeline that is configured to read single-end or paired-end FASTQ files. The best premium golf balls come in alternative versions that spin slightly less for players who prefer a firmer feel or more control off the tee. We recommend you try both kinds of feel from various distances to find your preferred feel. DNA nanoball sequencing technology offers some advantages over other sequencing platforms. One advantage is the eradication of optical duplicates. DNA nanoballs remain in place on the patterned array and do not interfere with neighboring nanoballs. Wilson offers two premium golf balls in its current range, the Staff Model and Staff Model R, both of which offer tour-proven performance. Both balls feature a four-piece construction and highly responsive urethane cover, with the result being distance and control in the long game and ample spin and soft feel on and around the greens. The primary difference between the two models is that the R version of the ball is unpainted, which Wilson says will deliver a more controlled trajectory, increased short game spin, and improved accuracy on full shots. Do any professional golfers use Wilson golf balls?

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The Wilson Staff Model is one of the best premium golf balls on the market and a ball that is being played on professional tours around the world. The Staff Model features a four-piece construction and soft urethane cover - a combination that delivers exceptional distance off the tee while providing players with maximum control and exceptional feel from 100 yards out. Huang, J. (2017). "A reference human genome dataset of the BGISEQ-500 sequencer". GigaScience. 6 (5): 1–9. doi: 10.1093/gigascience/gix024. PMC 5467036. PMID 28379488. CTAGGCAACTATAGGTCTCAGTTAAGTCAAATAAAATTCACATCAAATTTTTACTCCCACCATCCCAACACTTTCCTGCCTGGCATATGCCGTGTCTGCC java -jar picard.jar MarkDuplicates I=input.bam O=marked_duplicates.bam M=marked_dup_metrics.txt READ_NAME_REGEX=nullAbsolutely. Wilson might not have the brand recognition in the golf ball market that some other companies do but it has devoted significant R&D resources to its golf ball line and has a wide range of products to fit the needs of any player. Wilson has been especially successful in developing golf balls at lower price points to help recreational players on a budget still enjoy high-quality performance benefits from tee to green. That said, Wilson's top-of-the-line Staff Model franchise has consistently delivered premium balls that are played on professional tours across the globe and competitive with the most popular balls in the game. What is Wilson's premium golf ball?